When to collect urine for the test?Any urine specimen is appropriate for Pregnancy Testing but the first morning urine specimen is optimal because of its highest concentration of HCG.
How to collect urine?Urine specimens may be collected in any clean, dry, plastic or glass container.
How to do the test? 
1. The test strip and urine have to be at room temperature (15-30ºC) for testing.
2. Remove the test strip from the sealed pouch.
3. Immerse the strip into the urine with the arrow pointing towards the urine. Take the strip out after 3 seconds and lay the strip flat on a clean, dry, non-absorbent surface (such as the mouth of the urine container).
4. Read results in five minutes. Do not read results after more than 5 minutes.
Important: Do not allow the urine level to exceed the MAX (marker line), otherwise the test will not perform correctly.
How to read the resultsNegative (not pregnant) :
Only one color band appears in the Control Zone. No apparent band on the Test Zone. This indicates that no pregnancy has been detected.
Positive (pregnant) :
Distinct color bands appear in the Control and Test Zones. It indicates that you are pregnant. The color intensity of the test bands may vary since different stages of pregnancy have different concentrations of HCG hormone.
Invalid:
p HCG Urine Test
No visible band at all, or there is a visible band only in the test region and not in the control region. Repeat with a new test kit. If test still fails, please contact the distributor or the store, where you bought the product, with the lot number.
NOTE: if the color in the Test Zone is weak, it is recommended that the test be repeated in 48 hours.What to do when you know the resultIf the result is positive (two lines)
Consult your physician. He should confirm your pregnancy after evaluating all clinical and laboratory findings. Indeed, in certain conditions, the test may give a false positive result. He will also give you further information and advise on your pregnancy.
See also “Limitations”.
If the reult is negative (one line)
If you have reasons to believe that you are pregnant (e.g. no menstrual bleeding), you can repeat the test a few days later. If the test is still negative, consult your physician.
See also “Limitations”.
Limitations 1. As it is with any diagnostic procedure, a confirmed pregnancy diagnosis should only be made by a physician after evaluating all clinical and laboratory findings.
2. If a urine sample is too dilute (ie, low specific gravity) it may not contain a representative level of hCG. If pregnancy is still suspected, another urine specimen should be collected 48 hours later and tested.
3. Low concentration of hCG in a very early pregnancy can give a negative result. In this case, another specimen should be obtained at least 48 hours later and tested.
4. Elevated levels of hCG can be caused by a few conditions other than pregnancy. Therefore, the presence of hCG in urine specimen should not be used to diagnose pregnancy unless these conditions have been ruled out.
5. A normal pregnancy can not be distinguished from an ectopic pregnancy based on hCG levels alone. Also, spontaneous miscarriage may cause confusion in interpreting test results.
6. Sterility treatments, based on hCG, may cause false results. Consult your physician.